Sleep & Circadian Research Bundle x 8 Vials (Epitalon 50mg / Pinealon 10mg / DSIP 10mg)

A circadian or sleep study can be read on three levels, and each peptide in this set enters at a different one. Epitalon sits at the level of the clock signal, through its links to the pineal gland and the evening rise in melatonin. Pinealon sits at the level of the cell, with its research centred on how neurons cope with oxidative stress, hypoxia and amyloid damage. DSIP sits at the level of the brain-wave output, where its classic read-out is delta power on the EEG. That gives a combined model three separate entry points and lets hypotheses be framed precisely: does a shift in melatonin timing change delta activity on its own, and does protecting neurons from oxidative stress change how either of those measures responds?

Epitalon and Pinealon both come from Professor Vladimir Khavinson's short-peptide “bioregulator” programme, which proposes that peptides of two to seven amino acids can act inside the cell nucleus rather than at a surface receptor. DSIP has a completely separate history: it was found by a Swiss team led by Marcel Monnier and Guido Schoenenberger, and decades later still has no identified gene or confirmed receptor, a puzzle one review described as an unresolved riddle (Learn more). We are not aware of any published study testing two or all three of these peptides together, so each needs its own response curve in your chosen model before a combination arm means anything. Early animal work on DSIP also reported a U-shaped response, fading at both low and high concentrations (Learn more), which makes a proper concentration range especially important for that arm.

In rodent work, EEG and EMG recordings give delta power, non-REM and REM time and sleep latency, the measures on which DSIP was first defined. Melatonin and cortisol sampled at fixed points across the light–dark cycle give the circadian read-out most associated with Epitalon. In culture, neuronal or PC12 cells under oxidative challenge give reactive oxygen species, necrotic cell counts and ERK 1/2 activation, the measures used in the original Pinealon cell paper. Stress researchers can add c-Fos counts in the paraventricular nucleus of the hypothalamus, a marker DSIP reduced after immobilisation stress in rats (Learn more). Reading every arm on every measure is what turns three single-peptide experiments into a genuine comparison.

Equal milligrams do not mean equal numbers of molecules here. A 50mg Epitalon vial (about 390.35 g/mol) holds roughly 128 micromoles, a 10mg Pinealon vial (about 418.40 g/mol) about 24 micromoles, and a 10mg DSIP vial (about 848.81 g/mol) around 11.8 micromoles, so one Epitalon vial carries about five times the molecules of a Pinealon vial and roughly eleven times those of a DSIP vial, even though the Pinealon and DSIP vials hold the same 10mg. Comparisons between arms are therefore best set on a molar basis. DSIP is also the most delicate of the three, with a light- and oxidation-sensitive tryptophan at the start of its chain and a short life in biological fluids, so the three DSIP vials suit a one-vial-per-run pattern: each experimental series starts from freshly opened material.